6 Method Parameters
Additional Information
2D-LC User Guide 178
Figure 93 Example of High Resolution sampling (5 cuts) with 5 analytical gradients
Figure 94 Example of High Resolution sampling (5 cuts) with only one analytical gradient for
all cuts
Multi-Inject works similar to an injection from a large sample loop. Large
injection volumes can negatively affect
2
D separation. Consider a good
2
D
retention by starting at low percentages of B and by applying ASM. Therefore,
Multi-Inject is not recommend for volume-based isocratic separations, e.g. SEC.
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