Agilent Technologies 1290 Infinity II 2D-LC User Manual

2D-LC User Guide 211
8 Run the System
Run the Experiment
Table 22 Recommended conditions in 2D (HPLC) for high resolution
Parameter Value
2D-LC Valve
MHC with 40 µl sample, Transfer Capillary, ASM Factor No
2
D Column Compartment (MCT)
Column RRHD Bonus-RP, 2.1x 50 mm, 1.8 µm, 1200 bar (857768-901)
Column temperature 40 °C
Stop time As pump/No limit
2
D Pump
Heart Cutting (time or peak based)
Mobile Phase A Water + 0.2 % formic acid
Mobile Phase B Acetonitrile
Flow Rate 1 mL/min
Idle flow not used
Stop time 40 min (will not automatically prolonged, if peaks in 2D are not work off)
Post time 6 min
Sampling Table Start 11.15 min, 10 cuts, Cut Size 3.2
Figure 109 HiRes
The Cut-Time (HiRes) can vary slightly depending on the configuration and the used hardware.
2D Gradient: Analysis 1.25 min, Equilibration 0.50 min
Analytical gradient - Shifted Gradient Shift 1D:
10 % B 0.00 min - 30 % B 20 min
60 % B 1.25 min
Flush gradient 80 % B 0.00 min
+ 2 * column void volume
corresponds approximately to 0.21 min
2
D Detector (DAD)
Diode-array 254 nm, Bandwidth 4 nm
Reference Wavelength 360 nm
Reference Bandwidth 100 nm
Peak width 80 Hz
Stop time As pump/No limit

Agilent Technologies 1290 Infinity II 2D-LC Specifications

General

Brand	Agilent Technologies
Model	1290 Infinity II 2D-LC
Category	Measuring Instruments
Language	English