8 Run the System
Run the Experiment
2D-LC User Guide 218
1 Load method Comprehensive Checkout from the 2D-LC data media and
modify the settings for your Comprehensive configuration.
2 Run the method with 2D-LC starter sample, 1 x 2 mL (5190-6895), 1:10 (for
only UV Checkout), 1:100 (for LCMS Checkout), or 1:1000 (for LCMS
Checkout) diluted with Methanol/Water (20/80; v/v) with 0.1 % formic acid.
3 If necessary, subsequently edit or optimize the method.
Min Height 1000 counts
Reference Masses
Positive
121.05087300
922.00979800
Chromatograms
Chrom Type Label Offset Y-Range
TIC TIC 1510000000
TIC TIC 1510000000
Stop time As pump/No limit
Table 27 Recommended conditions in 2D (LC-MS)
Parameter Value
To avoid problems in the LC/MS due to the high flow rate the effluent from the second dimension
column should be split. The recommended split ratio is 1:2
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