Agilent Technologies 1290 Infinity II 2D-LC User Manual

2D-LC User Guide 221
8 Run the System
Run the Experiment
Sampling Table Start 0.2 min Time based, 2.5 min Peak Based, 4.50 min
2D Gradient Analysis 1.50 min Equilibration 0.50 min Cycle time 2.12 with ASM ON and ASM Factor 3
Analytical gradient - Shifted Gradient Shift 1D:
3% B 0.00min
3% B 0.37min
10 % B 0.38 min
60 % B 1.62
30 % B 6 min
2
D Detector (DAD)
Diode-array 254 nm, Bandwidth 4 nm
Reference Wavelength 360 nm
Reference Bandwidth 100 nm
Peak width 80 Hz
Stop time As pump/No limit
Table 29 Recommended conditions in 2D (HPLC) for ASM MHC 2D-LC
Parameter Value

Agilent Technologies 1290 Infinity II 2D-LC Specifications

General

Brand	Agilent Technologies
Model	1290 Infinity II 2D-LC
Category	Measuring Instruments
Language	English