2D-LC User Guide 223
8 Run the System
Run the Experiment
1 Load method ASM Multiple Heart-Cutting Checkout from the 2D-LC data media
and modify the settings for your multiple heart-cutting configuration.
2 Run the method with 2D-LC starter sample, 1 x 2 mL (5190-6895), 1:10 (for
only UV Checkout), 1:100 (for LCMS Checkout), or 1:1000 (for LCMS
Checkout) diluted with Methanol/Water (20/80; v/v) with 0.1 % formic acid.
3 If necessary, subsequently edit or optimize the method.
Min Height 1000 counts
Reference Masses
Positive
121.05087300
922.00979800
Chromatograms
Chrom Type Label Offset Y-Range
TIC TIC 1510000000
TIC TIC 1510000000
Stop time As pump/No limit
Table 30 Recommended conditions in 2D (LC-MS)
Parameter Value
Adjust the ASM split ratio
To optimize the ASM split ratio of the method either for highest resolution
(strong dilution), or lowest cycle time (weak dilution), different ASM capillaries
are available.
The checkout method uses ASM factor 3, see “Understanding the ASM
Factor” on page 42.
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