2D-LC User Guide 227
8 Run the System
Run the Experiment
Table 34 Recommended conditions in 2D (LC-MS)
Parameter Value
Ion Source Atmospheric pressure electrospray (Dual AJS ESI)
Ion Mode Dual AJS ESI
Ion polarity Both
Storage Mode Both
LCMS Stream MS
Acquisition Mode Acquisition Mode MS1 Min Range (m/z) 50, Max Range (m/z) 1000, Scan
Rate (spectra/sec) 3.00
Instrument Parameters
Gas Temp 250 °C
Gas Flow 11 L/min
Nebulizer 40 psig
SheathGasTemp 350 °C
SheathGasFlow 12 L/min
Scan Segment 1
Scan Source Parameters
Vcap 3500 V
Nozzle Voltage 300 V
Fragmentor 120
Skimmer1 45
OctopoleRFPeak 750
ReferenceMasses
Ref Mass Enabled Enabled
Use Bottle A
RefNebulizer
True
Ref Nebulizer 0 psig
To avoid problems in the LC/MS due to the high flow rate the effluent from the second dimension
column should be split. The recommended split ratio is 1:2
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