2D-LC User Guide 273
9 Data Analysis
GC Image Basic Information
6 Apart from using continuously shifted gradients in the second dimension, as
was done during the checkout runs, it is also possible to stepwise shift the
second dimension gradients. For this purpose, keep the valve & loop
configuration as well as the 1D pump method the same. In Instrument > Setup
2D-LC, set up a
2
D pump and modulation method with stepwise shifted
gradients as shown below:
7 Run the comprehensive 2D-LC analysis with stepwise shifted gradients in the
second dimension.
The resulting separation should look similar to the one shown below:
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