2D-LC User Guide 29
2 Concepts of 2D-LC
Multiple Heart-Cutting and High Resolution Sampling 2D-LC
High-resolution sampling (time-based mode)
For high-resolution sampling, a (start) time can be set, the cut size in seconds
and the number of cuts for a peak or range. The sampling time should be less
than the time which is needed for filling one sample loop corresponding to a loop
filling below 80%. Because of the parabolic flow profile, a filling greater than 80%
will cause samples going to waste.
The minimum cut size is given by the transfer volume between the 2D-LC valve
and the deck valve. The last cut of a deck is stored in the transfer capillary such
that switching to the second deck will bring that peak to the second dimension. If
a volume smaller than that transfer volume would be chosen, two cuts would be
in the same capillary resulting in a loss of resolution and reproducibility.
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