2D-LC User Guide 31
2 Concepts of 2D-LC
Comprehensive 2D-LC (LCxLC)
Standard LCxLC
In standard LCxLC the total eluent of the first dimension is injected onto the
column in the second dimension using two sampling loops alternatingly by
switching a modulation valve. This will be repeated from the start to the end of
the first dimension separation.
Figure 8 Principle of standard LCxLC
D chromatogram
1
D sampling
D gradient
2
2
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