14 Theoretical Background
Theoretical basis of 2D-LC
2D-LC User Guide 366
2D-LC In 2D-LC the separation behaviour is more complex and described below.
Figure 171 Diagram of
2
D resolution measurement: Slice for resolution (top) and 2-dimensional
contour plot (bottom)
The distance between two spots in the contour plot may be calculated by the
Pythagorean expression:
For the resolution along the axis of each dimension applies:
and
So for two dimensions the resolution may be calculated as follows:
Figure 172
2
D resolution (pythagorean relation)
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