2D-LC User Guide 6
5 2D-LC Data Acquisition in MassHunter Workstation 11 103
Start the Data Acquisition Software 104
Overview 2D-LC in MassHunter Acquisition 11 106
Instrument Status 107
2D-LC User Interface 108
2D-LC Valves Online Monitor in the 2D-LC User Interface 114
Method Editor Window 116
Sample Run Window 117
Worklist Window 118
Tune Window 119
Instrument Details 120
Log book in MassHunter Acquisition 11 123
6 Method Parameters 125
Method Editor 2D-LC 126
Set the 2D-LC Method parameters 127
Preview (2D-LC) 154
Set up a Peak-Based Experiment Graphically 168
Setup 2D Gradient Graphically 173
Setup Second Dimension Gradient with the Graphical User Interface 175
Additional Information 177
7 Method Development of Active Solvent Modulation (ASM) 185
Method Development of Active Solvent Modulation (ASM) 186
Method Parameters 187
Optimize the Dilution by Using ASM Capillaries 188
Optimize the Sample Loop Flush 189
Include the ASM Phase to the 2D Gradient 190
Optimize Dilution Through Method Settings 191