Agilent Technologies 4150 TapeStation system User Manual

Agilent 4150 TapeStation System Manual 179
Troubleshooting
10
Troubleshooting DNA Applications
Sizing
Genomic DNA Ladder should be freshly prepared for each run. No electronic
ladder is available for the Genomic DNA assay. The ladder vial should be
handled carefully with only gentle vortexing before use. Shaking the Genomic
DNA ladder vial can occasionally cause the largest fragment to degrade, since
shaking results in different shearing forces than vortexing. This can lead to
inaccurate sizing results. Where the degradation has resulted in failure of the
software to assign the top ladder fragment, no DIN scores will be presented for
the lanes in this file.
Figure 45 The effect of vigorous shaking of the Genomic DNA Ladder. A) Genomic DNA
Ladder has been handled as directed prior to analysis on the Genomic DNA
ScreenTape assay. B) The Genomic DNA Ladder vial was handled incorrectly -
degradation of the top fragment (48500 bp) is clearly shown.
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Agilent Technologies 4150 TapeStation system Specifications

General

Brand	Agilent Technologies
Model	4150 TapeStation system
Category	Laboratory Equipment
Language	English