Agilent 4150 TapeStation System Manual 209
Troubleshooting
10
Troubleshooting RNA Applications
Additional bands have
appeared to those
expected in the sample
lane
There may be a
contaminant peak that
is caused by dust or dirt
on the ScreenTape
device
Peaks caused by
contaminants appear
as sharp strong bands
which are often slanted
or uneven in the gel
image, and are usually
easily distinguishable
from sample peaks
Unalign the image using the Aligned button in
the TapeStation Analysis software. Contaminant
peaks can be unassigned by right-clicking on
either the gel image or electropherogram peak.
Rerun samples if needed
A bubble could be
located within the gel
matrix
A bubble in the gel
matrix will introduce
significant artifacts in
the data. Those bubbles
cannot be flicked away
Always inspect ScreenTape devices for bubbles
in the lanes. Lanes in question cannot be used
for analysis. Such bubbles can form after the
expiration date or upon wrong storage. See the
Agilent Information Center and “ScreenTape
Devices” on page 87 for more information
Fragmented genomic
DNA contamination
Genomic DNA is a
common contamination
in total RNA samples.
Genomic DNA might be
subject to apoptotic
laddering. It is the
result of natural DNA
fragmentation
processes occurring
during apoptosis. See
the Agilent Information
Center for more
information.
Treat the RNA sample with DNAse to remove
contaminating DNA from the sample
Reagent Buffer or
sample contaminated
with ladder
Failure to change
pipette tips in between
pipetting steps might
lead to Reagent Buffer
or sample being
contaminated with
ladder.
Use new Reagent Buffer vial and make sure to
change pipette tips in between pipetting steps.
See “Sample Preparation” on page 91 for more
information
Table 16 RNA - Possible causes of unexpected migration profile.
Problem Likely Cause Explanation Solution
To Next Page
Loading...
Need help?
General
