6 Agilent 4150 TapeStation System Manual
Contents
6 Operating Instructions 63
Sample Preparation 64
Preparing the Instrument 65
Starting the Analysis 73
Analysis End 77
Follow Up 78
7 Good Measurement Practices 81
Use of Correct Tools 83
Tools and Handling 84
Good Pipetting Practice 85
Reagents and Reagent Mixes 86
ScreenTape Devices 87
Partially Used ScreenTape Devices 90
Sample Preparation 91
Ladder Considerations 92
Use the Correct Protocol 93
Mixing Recommendations 94
Centrifugation Recommendations 97
RNA Assays 99
Peak Integration 101
Peak Maxima Versus Average Molecular Weight Sizing and Molarity 104
Sizing Range 106
Quantitative range 107
Maximum Buffer Concentration in Sample 110
RINe Functional Range 112
8 Instrument Diagnosis 113
Instrument Diagnosis 114
Tests 115
Reporting 122