Preventive Maintenance and Troubleshooting
Chromatographic symptoms
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Chromatographic symptoms
You may suspect a problem in the automatic liquid sampler based on what you
see in a chromatogram or by comparing chromatograms. Each of the five parts
of this section is named after a chromatographic symptom. Each part contains
a list of possible causes and recommended solutions.
It is often difficult to identify a cause. For this reason, the causes are listed
from most common to least common.
If you cannot correct the problem, obtain Agilent service.
Variability
Figure 68. Retention times or areas are not reproducible
Inlet septum is leaking
If the septum is leaking, replace the septum. If the leaking septum has
experienced less than 200 injections, check for the following:
• The septum retainer nut is too tight. See “Changing septa” on page 54.
• The syringe needle is not straight. See “Inspecting syringes” on page 51.
• The syringe is not installed correctly. See “Installing syringes” on page 52.
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