Preparing for Operation
Selecting and installing syringes
51
Figure 38. Injection volumes depend on sample volume setting, syringe size and
injection type
Caution Failure to use an on-column syringe when injecting into an on-column inlet
could damage the injector, syringe and column.
Inspecting syringes
Before installing the syringe:
1. Roll the syringe on the edge of a clean flat surface. If the tip of the needle
seems to follow a circular motion, bend it slightly near where it connects
to the syringe barrel, and check it again.
Figure 39. Syringe parts and needle inspection
2. Check for a rough needle. The needle may contain closely spaced
concentric ridges that act like a miniature file and abrade pieces of the
septum into the inlet. The ridges are easy to see under 10X magnification.
Sample Volume Setting Standard Injection With Nanoliter Adapter
Syringe
Size
5 µl 10 µl 5 µl 10 µl
1 0.5 1.0 0.1 0.2
2 1.0 2.0 .05 1.0
3 1.5 3.0 1.0 2.0
4 2.0 4.0 1.5 3.0
5 2.5 5.0 2.0 4.0
Bend here if necessary
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