Special Topics
Performing cool on-column injection onto 250-µm and 320-µm columns
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each other inside the ferrule during tightening, exposure to ferrule material is
minimized.
Purged connectors
Purged connectors are commercially available for column connection. The
most complex of connector types, they purge the connection area and, thus,
minimize contamination.
Preparing the injector
The preparation of the injector is divided into the following steps:
• How to change the needle support assembly
• How to install the syringe into the needle support assembly
Changing the needle support assembly in the injector
When changing to 250-µm or 320-µm injection, you must change the needle
support assembly. The needle support assembly is a spring-loaded rod with a
needle support foot at its base. The foot supports and guides the needle during
injections.
Removing the needle support assembly
1. Lay the injector module on its back on a flat surface.
2. Open the injector door.
3. Loosen the plunger screw. (The plunger screw holds the plunger in the
plunger carrier.)
4. Slide the loop of the plunger carrier up as far as it will go.
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