Preparing for Operation
Preparing the solvent and waste bottles
37
• For many common solvents, the rate of diffusion out of the bottle is less
with a diffusion cap than with a septum that has been punctured with a
standard syringe needle.
Figure 27. 4-ml bottle used for solvent and waste
Filling and placing the bottles
Before each sequence or group of sequences, prepare your solvent and waste
bottles by doing the following:
1. Rinse and fill each solvent bottle with 4 to 4.5 ml of fresh solvent. The
liquid level should be near the shoulder of the bottle. If the solvent bottle is
filled with 4.5 ml of solvent, the syringe can reach about 2 ml or about 250
washes for a 10-µl syringe.
Figure 28. Shows position of the syringe tip when withdrawing solvent
Diffusion cap
Agilent Technologies recommends
using a diffusion cap instead of a
septum to reduce solvent
contamination and evaporation.
4.5 ml maximum
2.5 ml minimum
18.5 mm
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