Preparing for Operation
Preparing the solvent and waste bottles
36
7. Restore the power. The fan should now be off. If it is still running, reopen
the injector, and check the position of the P6 jumper according to the
instructions in step 5.
Preparing the solvent and waste bottles
The solvent bottles hold solvent for rinsing the syringe between injections. The
injector dispenses the solvent washes and sample washes into waste bottles.
The first two parts of this section explain how to select, fill, and place the
solvent and waste bottles. The last two parts of this section explain how to
estimate the maximum number of vials you can run at one time and how the
injector controls carryover.
For information on how to set the number of washes, see “Setting the run
parameters” in chapter 3.
Selecting the bottles
The injector uses 4-ml bottles with diffusion caps to hold the solvent and
waste. You can use diffusion caps or septa on these bottles to reduce
evaporation and diffusion of your solvents and waste. Agilent Technologies
recommends diffusion caps over septa for two reasons:
• The diffusion cap allows multiple entrances into a bottle without
contaminating the liquid inside the bottle with small pieces of septum
material.
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