Special Topics
Performing cool on-column injection onto 250-µm and 320-µm columns
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Performing cool on-column injection onto 250-µm and
320-µm columns
Installing the needle into the syringe barrel
The stainless steel needles used for 250-µm and 320-µm injections must be
inserted into a glass syringe barrel (part no. 5182-0836 [5 µl]). Select the correct
needle for the column you will use. Needles for 250-µm injections (part no.
5181-7442) have silver-colored stops. Needles for 320-µm injections (part no.
5181-1266) have gold-colored stops.
1. Unscrew the syringe barrel cap, and remove the spring.
2. Make sure the needle has the Teflon disk as shown in the picture below. If
the syringe barrel does not have the Teflon disk, use the instructions in the
syringe box to wrap the needle yourself.
3. Slide the spring and the cap down over the needle.
4. Insert the needle into the syringe barrel.
5. Screw the cap back on the syringe barrel.
Figure 73. Syringe parts and assembly
Needle
Spring
Cap
Stop
Stop
Needle
Syringe barrel Teflon disk
Teflon disk
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