Special Topics
Performing cool on-column injection onto 250-µm and 320-µm columns
167
Troubleshooting 250-µm and 320-µm systems
Symptom Possible Cause Corrective or Preventive Action
Bent needle Incorrectly installed needle
support assembly
Check needle support assembly installation.
Defective needle Check each syringe before installation to make sure the needle is
straight.
Incorrect insert Make sure the insert is the correct size for the column and needle you
use. Also check that the insert is installed correctly.
Worn or damaged needle guide Check the needle guide on the needle support foot every time you
change the inlet septum, and replace if necessary.
Incorrect inlet septum Use only a 5-mm septum with a through-hole.
Poor alignment of inlet septum
and septum nut
Align the inlet septum and septum nut according to the instructions
provided in this manual.
Incorrect column internal
diameter
Check the internal diameter of the column by using the appropriate
insert.
Closed inlet septum hole Replace the septum.
Poor alignment of the inlet and
the automatic injector
See the 5890 Series II Operating Manual for alignment instructions.
No peaks or
unexpectedly
small peaks
Plugged syringe needle Replace the needle, or clean it with wire.
Worn syringe barrel Replace the syringe often, or use a Teflon-tipped plunger.
Loose removable needle Make sure the syringe barrel caps are screwed on tightly and that the
Teflon disk is in place.
Incorrectly placed or missing
Teflon disk on the syringe
needle
Check every syringe needle to make sure the Teflon disk is present
and correctly placed.
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