Special Topics
Performing cool on-column injection onto 250-µm and 320-µm columns
168
Controlling sample vial temperatures
This section explains how to connect tubing to the tray quadrants, how to
calculate the water bath temperature used to obtain an approximate sample
vial temperature, and the pressure limitations of the quadrants.
This section does not explain how to set up a water bath and pump.
You can control the temperature of the sample vials in the tray by:
• Pumping a temperature-controlled liquid through the tray quadrants.
• Freezing an ethylene glycol and water solution inside the tray quadrants.
The quadrants are designed for low flow rates, low pressures, and long
temperature equalization times. If you freeze an ethylene glycol or alcohol
solution in the quadrants, fill the quadrants to less than 90% of their total
capacity.
Symptom Possible Cause Corrective or Preventive Action
Poor precision,
poor repeatability,
or large standard
deviation
Worn syringe barrel Replace the syringe.
Loose removable needle Make sure the syringe barrel caps are screwed on tightly and that the
Teflon disk is wrapped tightly as shown in this chapter.
Widened holes in vial caps Replace vial caps when holes widen and leaks develop.
Inlet pressure set too low Adjust the pressure.
Incorrectly placed or missing
Teflon disk on the syringe
needle
Check every syringe needle to make sure the Teflon disk is present
and correctly placed.
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