Glossary
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The third chromatogram shows the result of washing the syringe with four
solvent washes. The carryover peaks disappear.
septum bleed: the inherent baseline noise level emanating from a column due
to decomposition or volatilization of low molecular weight products mainly
from the stationary phase. The septum is another source of volatiles that cause
noise.
septum life: usually refers to the number of injections a septum survives
before it begins to leak.
Carryover peaks
Sample #1: 20 mg/ml
of solute in methanol
Sample #2a: Methanol
blank without washes
Sample #2b: Methanol
blank after 4 washes
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