Preparing for Operation
Adapting for cool on-column injection
56
• Slows the plunger speed to 1/18 the normal speed
Complete the following:
1. Install an on-column syringe. Agilent Technologies recommends syringes
with a 23/26 gauge tapered-tip needle for injections onto 530-µ columns
and 32/26 gauge for 320- and 250-µ columns.
2. If the injector is controlled by an external device, such as an integrator or
a workstation, change the on-column parameter to ON or YES.
If the injector is controlled by a 6890 GC, this will be set automatically
when the GC configures for the on-column inlet.
If the injector is operated with standalone control, change the on-column
switch to the YES, or down, position. For more information on the on-
column switch, see “On-column injection mode” in chapter 4.
3. The inlet must be configured for cool on-column injection. Install the
appropriate needle guide, septum, spring, and insert for the GC.
If you have an on-column inlet on a 5890A GC with a cooling tower
(part no. 19245-20540), replace it with a septum nut base assembly
(part no. 19245-80520).
If you are performing automated injection onto 320-µm and 250-µm
columns, replace the standard septum nut base assembly (part no.
19245-80520) with the small septum nut base assembly (part no.
07673-61330)
4. For injecting onto 320-µm or 250-µm columns, see “Performing cool on-
column injection onto 250-µm and 320-µm columns” in chapter 6.