Measurement with the cell changer
Carrying out measurements with the cell changer
SPECORD S600 accessories Edition 09/2014 35
Kinetic measurements with the cell changer
The cell change during kinetic measurements takes place in two different
modes adapted to the reaction speed.
Mode for fast reaction kinetics
Mode for slow reaction kinetics
Fig. 14-2 Modes for the cell change during kinetic reactions
Mode for fast reaction kinetics
For fast kinetics the complete reaction in a cell is tracked. This is followed by a
change to the next cell and the reaction start in this cell. Each cell is processed
in this way.
The measuring start in each cell takes place via a separate start command
(Start request) to have enough time to add the start reagents to the sample.
In this type of cell change up to four intervals with different measuring times can
be configured.
Example for the measuring parameter configuration for an adapter of fast
kinetics using a 6-cell changer:
Mode for slow reaction kinetics
For slow kinetic reactions a measurement is carried out in each cell as part of
a cycle. During the next cycle the measurements are repeated in each cell,
which continues until the end of the total measuring period.
Kinetics can be recorded at fixed wavelengths or by scanning an entire
spectrum. The following must be considered in the measuring parameter
configuration:
Mode tab
Parameter Settings
Time scan
Measurements are recorded at fixed wavelengths.
Choose the desired wavelengths from the list box.
The settings for kinetics are made on the Mode tab.
Spectrum
An entire spectrum will be scanned.
The settings for kinetics are made on the Interval tab.
Mode tab or Interval tab
When scanning an entire spectrum select option Time controlled on the
Interval tab.
Start request
When activated the measuring start in each cell takes
place via a separate start command.
Waiting time
If necessary, set period before commencing the first
measurement in the sample series.
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