Analysis of light-scattering samples
Integrating sphere
SPECORD S600 accessories Edition 09/2014 93
3. Put the control unit and the sphere body into the sample compartment of
the SPECORD S 600 according to the above instructions. Make sure that
the sphere body is moved fully right up to the stop.
4. Establish the electric connections between control unit, sphere body and
transformer. Turn the lamp on.
Transmittance measurement procedure
1. Switch on the SPECORD S600 and start WinASPECT.
2. Enter the respective parameters and determine the integration time without
cell.
3. Then, take a reference measurement. Decide on the use of a reference
substance depending on your specific analytical task.
4. Insert the sample cell in the sphere body. Consider that the sphere body
must be in the same position as that used for the reference measurement.
5. Start the measurement in the usual way.
If you want to take a very accurate transmittance measurement at a defined
wavelength, calibrate the device in the same way as in Quantitative Analysis
using several certified transmission standards. Subsequently, as you are used
to do from concentration determination in Quantitative Analysis, measure the
unknown sample versus the calibration curve just determined.
Before the determination of the integration time, you must not have run a dark
current me
asurement. If so, re-initialize the device.
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