HT2000H_en_n.doc Page 100 of 167
• Injection: see paragraph 5.2.2.5;
Tap “Exit” to exit from the “METHOD X” screen.
If one of these parameters has been changed a confirmation screen appears “Modifying Method
X”. Tap “SAVE” to save the modifications or “CANCEL” to exit without saving. The display will
return to the “RUN: METHODS” screen.
5.2.2.1 General parameters
Access sequence: RUN>SETTINGS>METHODS>METHOD X>GENERAL
The following parameters can be viewed/edited:
• Syringe Volume: volume of the syringe. The syringe volume affects the limits of other
parameters such as speed and volume;
• Analysis Time: time of the sample analysis (for details see below).
• Method Type: parameter not editable;
• Preparation Time: displays how long the autosampler needs to perform a cycle. It is
calculated from the instrument and is not editable (Time needed to transfer the vial from the
tray to the incubator, filling time, injection time, etc.).
Select “Exit” to exit from this screen and return to the “RUN: METHOD X” screen (see paragraph
5.2.2 “Method X: Setting method parameters”).
Analysis time
The Analysis time is the GC run time plus any additional time needed for the GC to execute any
post-run program and then return to a Ready state.
Please consider that the autosampler uses the Analysis time (GC cycle time) to calculate
throughput and timing. An accurate Analysis time is crucial to optimizing throughput and for
correctly processing samples.
If the Analysis time is too long, this can cause lowered throughput because vials wait longer than
needed before processing. If the Analysis time is too short, this can cause sequence faults
because the vial may sit too long while waiting for the GC to become Ready. It is better to enter a
longer time than needed than to enter too short a time and possibly reduce sample quality.
To determine the Analysis time, program the GC to perform a sequence of two or three blank (no
injection) runs. If you are using a Data system, you can determine the cycle time from the data
system sequence log and compare the start times for the runs. A good Analysis time is the average
difference between the start times, plus 0.2 to 0.5 minutes. If you are not using a Data system,
watch the GC. Count the time between the start of the first run and when the GC becomes Ready
for the second run, then add 1 minute.
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