HT2000H_en_n.doc Page 102 of 167
• Syringe Temperature: temperature of conditioning of the syringe. This value can be
between 40°C and 150°C (standard version syringe)/250°C (HT version syringe installed on
HT2000HT) or off (inactive conditioning);
• Oven Temperature: temperature of conditioning of the oven. This value can be between
40°C
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and 170°C (for HT2000H)/ 150°C (for HT2100H)/ 300°C (for HT2000HT) or off
(inactive conditioning);
• Incubation: sample incubation time inside the oven;
• Shaker On: interval time during incubation time in which the sample is shaken;
• Shaker Off: interval time during incubation time in which the sample is not shaken.
Warning
Do not set an oven temperature lower than the ambient temperature. It could provoke an oven
error (see paragraph 8.13 “Oven error“).
Select “Exit” to exit from this screen and return to the “RUN: METHOD X” screen (see paragraph
5.2.2 “Method X: Setting method parameters”).
5.2.2.4 Sample parameters
Access sequence: RUN>SETTINGS>METHODS>METHOD X>SAMPLE
The following parameters can be viewed/edited:
• Sample Volume: volume of sample to be injected;
• Fill Volume: the volume that the syringe dispenses inside the vial if the “Syringe Prefill“ has
been selected as “YES”, or the volume that the syringe aspirates and dispenses from the
vial during the Pull Up Strokes (if the Pull Up Strokes number set is different from zero);
• Pull Up Strokes: the number of pre-aspirate strokes (during each stroke the “Fill Volume”
is aspirated and ejected). This procedure assures a good mix of the sample.
• Equilibration Delay: delay time between sample pick up and the syringe moving from the
sample;
• DHS Time: to be set for special application only;
• Sample speed: speed of the plunger during the syringe filling operation;
• Syringe Prefill: syringe-filling mode. You can choose if, when the needle comes in the vial,
the syringe plunger has to be pulled up (YES) or pushed down (NO), before the septum
has been pierced.
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Or the minimum oven temperature that can be set in your special autosampler version
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