2D-LC User Guide 145
6 Method Parameters
Set the 2D-LC Method parameters
Modify the Solvent Composition in the
2
D gradient Over the
Run Time of
1
D
Use this section to modify the solvent composition in the
2
D gradient over the run
time of the first dimension. For each setpoint in the Analytical Gradient table that
is marked in the Shift column, you can set up a gradient shift as a nested
gradient. The gradient shift is used to align the
2
D gradient composition with the
1
D gradient composition.
Use the table to set up the shifted
2
D gradient
1 To set up the shift gradient, select the corresponding line in the Analytical
Gradient table.
2 Specify the time for the change of solvent composition in the Time[min] field.
The shifted gradient composition changes linearly from one setpoint to the
next. Change the solvent composition at a specified time. The time axis
relates to the stop time of the
2
D pump, a time greater than stop time
2
D will
be ignored.
3 Specify Percent B ranges from 0 – 100 % in the B[%] field.
Change the solvent composition at a specified time. Channel A always
delivers the remaining volume, %A = (100 - %B). The solvent composition
changes linearly from one setpoint to the next.
Different start conditions in the first row may cause step gradients and RI-effects
(density differences of the different liquid phases may cause different DAD
detection through baseline disturbances).
The selected shift check box in the analytical gradient window can only be
deactivated by removing the corresponding event in the Gradient Shift
1
D Time
window.
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