6 Method Parameters
Preview (2D-LC)
2D-LC User Guide 156
Finally loaded the data file that can be used to display a
1
D reference
chromatogram in the Preview.
Uploading a reference signal into the method screen can be helpful to illustrate,
at which positions of the chromatogram which cuts will be taken.
Figure 79 Loaded
1
D chromatogram in preview window
To cut the peaks correctly, the conditions, such as the flow under which the
reference chromatogram was recorded, must be maintained.
If further
1
D detectors are used as reference chromatogram, the transfer volume
must be corrected for these detectors. To do so, enter the shift volume or the
shift time.
If only one
1
D detector is configured, the transfer volume is already defined in the
2D-LC cluster and therefore does not need to be corrected here.
Shift time/Shift volume allows correction between cutting time at valve and
detection time. This correction might be necessary if not transfer volume is
defined in the 2D-LC cluster (see page 2D-LC Cluster). An example is the
Switchable
1
D/
2
D Setup where
1
D Q-TOF data are used as reference
chromatogram.
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