Agilent Technologies 1290 Infinity II 2D-LC User Manual

8 Run the System
Run the Experiment
2D-LC User Guide 208
2D Gradient: Analysis 1.25 min, Equilibration 0.50 min
Analytical gradient - Shifted Gradient Shift 1D:
10 % B 0.00 min - 30 % B 20 min
60 % B 1.25 min
Flush gradient not used
2
D Detector (DAD)
Diode-array 254 nm, Bandwidth 4 nm
Reference Wavelength 360 nm
Reference Bandwidth 100 nm
Peak width 80 Hz
Stop time As pump/No limit
Table 19 Recommended conditions in 2D (HPLC) for multiple heart-cutting
Parameter Value
Table 20 Recommended conditions in 2D (LC-MS)
Parameter Value
Ion Source Atmospheric pressure electrospray (Dual AJS ESI)
Ion Mode Dual AJS ESI
Ion polarity Both
Storage Mode Both
LCMS Stream MS
Acquisition Mode Acquisition Mode MS1 Min Range (m/z) 50, Max Range (m/z) 1000, Scan
Rate (spectra/sec) 3.00
Instrument Parameters
Gas Temp 250 °C
Gas Flow 11 L/min
Nebulizer 40 psig
SheathGasTemp 350 °C
SheathGasFlow 12 L/min
Scan Segment 1
Scan Source Parameters
Vcap 3500 V

Agilent Technologies 1290 Infinity II 2D-LC Specifications

General

Brand	Agilent Technologies
Model	1290 Infinity II 2D-LC
Category	Measuring Instruments
Language	English