Agilent Technologies 1290 Infinity II 2D-LC User Manual

2D-LC User Guide 209
8 Run the System
Run the Experiment
1 Load method Multiple Heart-Cutting Checkout from the 2D-LC data media and
modify the settings for your multiple heart-cutting configuration.
2 Run the method with 2D-LC starter sample, 1 x 2 mL (5190-6895), 1:10 (for
only UV Checkout), 1:100 (for LCMS Checkout), or 1:1000 (for LCMS
Checkout) diluted with Methanol/Water (20/80; v/v) with 0.1 % formic acid.
3 If necessary, subsequently edit or optimize the method.
Nozzle Voltage 300 V
Fragmentor 120
Skimmer1 45
OctopoleRFPeak 750
ReferenceMasses
Ref Mass Enabled Enabled
Use Bottle A
RefNebulizer
True
Ref Nebulizer 0 psig
AutoRecalibration
Average Scans 1
Detection Window
(ppm)
100 ppm
Min Height 1000 counts
Reference Masses
Positive
121.05087300
922.00979800
Chromatograms
Chrom Type Label Offset Y-Range
TIC TIC 1510000000
TIC TIC 1510000000
Stop time As pump/No limit
Table 20 Recommended conditions in 2D (LC-MS)
Parameter Value

Agilent Technologies 1290 Infinity II 2D-LC Specifications

General

Brand	Agilent Technologies
Model	1290 Infinity II 2D-LC
Category	Measuring Instruments
Language	English