2D-LC User Guide 39
2 Concepts of 2D-LC
Active Solvent Modulation (ASM)
Figure 21 Operating principle with sample loop flow path
Once the ASM phase has finished, which is a settable method parameter, the
analytical gradient starts. As opposed to a dilution with a permanent by-pass, the
ASM capillary is no longer in the flow path, such that fast
2
D gradients are
possible through the sample loop only.
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