Agilent Technologies 4150 TapeStation system User Manual

Agilent 4150 TapeStation System Manual 211
Troubleshooting
10
Troubleshooting RNA Applications
Low signal intensity of
sample or marker peaks
(one or other, not both)
Insufficient Mixing Buffer and sample was
not sufficiently
vortexed. The dye could
not incorporate
completely into the
sample
See “Mixing Recommendations” on page 94
and the Agilent Information Center for more
information
Incorrect ScreenTape
device or reagent
storage
Incorrect storage (too
cold or too warm) may
accelerate aging of the
ScreenTape device and
reduce the shelf life.
Storing reagents
incorrectly may induce
degradation of the dye.
Both can lead to a
higher than normal
background noise
Follow the storage conditions that are specified
for the assay (see Ta ble 3 on page 64)
Sample concentration
outside recommended
range for the
application
Linear concentration
estimation cannot be
achieved for samples
exceeding the limits in
the specification table
Either dilute or concentrate the sample until it is
within the recommended range for the
application as stated in the respective assay
Quick Guide (see “Quantitative range” on
page 107); then prepare a new run. Use the
High Sensitivity assay if applicable
Low signal intensity of
sample and marker
peaks (both) or of
ladder peaks
Incorrect reagent
storage
Storing reagents
incorrectly may induce
degradation of the dye.
This can lead to a
higher than normal
background noise
Follow the storage conditions that are specified
for the assay (see Ta ble 3 on page 64)
Incorrect migration -
samples have not
reached the lower end
of the gel lane
Salt concentration High salt
concentrations can
cause short running
within the gel lane
which can cause
incorrect identification
of Lower marker peaks
(see “Maximum Buffer
Concentration in
Sample” on page 110)
Unalign the image using the Aligned button in
the TapeStation Analysis software. This will
show the true position of all peaks present.
Please refer to the salt tolerance guidelines for
the assay (see Ta bl e 3 on page 64)
Partial electrophoresis
failure that is caused by
bubbles at gel-buffer
interface
Air bubbles at the
gel-buffer interface may
disrupt current flow
between the electrodes
Ensure ScreenTape device is flicked to remove
any bubbles before use. See the Agilent
Information Center and “ScreenTape
Devices” on page 87 for more information
Table 16 RNA - Possible causes of unexpected migration profile.
Problem Likely Cause Explanation Solution

Agilent Technologies 4150 TapeStation system Specifications

General

Brand	Agilent Technologies
Model	4150 TapeStation system
Category	Laboratory Equipment
Language	English