Special Topics
Performing cool on-column injection onto 250-µm and 320-µm columns
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8. Replace the spring on top of the insert.
Figure 76. Inlet assembly
You will align and install the inlet septum nut in the next section.
Aligning the septum nut
For 250-µm and 320-µm on-column injections, the septum nut requires a 5-mm
through-hole septum.
1. To ensure proper alignment, thread the septum and the septum nut onto a
26-gauge or larger needle or a piece of wire. The septum should rest in the
cavity in the nut.