Special Topics
Performing cool on-column injection onto 250-µm and 320-µm columns
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2. Turn the needle over. Insert the wire, inlet septum nut and through-hole
septum into the inlet.
Figure 77. Ensuring proper alignment
3. Tighten the septum nut as tight as possible. If the septum nut is loose, the
system may leak.
4. Remove the needle from the inlet.
Installing a fused silica capillary column into the inlet
You must cut the column correctly to ensure effective connection. The method
shown below produces the recommended square cut.
Needle
This end is
inserted into
the inlet
Inlet septum nut
This end is
inserted into
the inlet
Step 1 Step 2