Special Topics
Performing cool on-column injection onto 250-µm and 320-µm columns
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WARNING Flying glass particles can cause eye injuries. Always wear safety glasses when
cutting fused silica columns.
Figure 78. Cutting the column
1. Slide a column nut and ferrule onto the end of the column.
2. Cut off the column end with a square cut according to the illustration
above.
3. Wipe the column end with methanol to remove oil and dust.
4. Insert the column into the inlet base until it stops against the insert.
5. Finger-tighten the column nut. Make sure the column remains against the
insert while you tighten the nut.
6. Use a wrench to tighten the column nut an additional quarter turn.
7. Once you have selected the syringe, verify the column installation by
manually pushing it into the inlet. There should be a gap of 12 mm or less
between the septum nut and the syringe barrel. If there is more than
13 mm between the syringe barrel and septum nut, your needle is not
reaching the column, and you cannot perform 250-µm or 320-µm
3. Press to break.
1. Score the column with a
carbide knife.
2. Support opposite score
mark on knife edge.
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