Special Topics
Performing cool on-column injection onto 250-µm and 320-µm columns
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3. Pass the syringe needle through the hole of the small needle guide in the
needle support foot as shown below.
Figure 83. Needle support for 250-µm injection
4. Align the syringe flange with the flange guide and syringe clip, and gently
press the syringe into place, keeping the needle in the hole of the needle
guide. Be careful not to bend the needle during this step.
5. Close the syringe latch by swinging it clockwise.
Caution Do not operate the injector without a syringe in place because the syringe latch
may interfere with the motor if it is allowed to swing freely.
6. Move the plunger carrier loop down, and tighten the plunger screw.
7. Move the loop of the plunger carrier up and down to make sure the
plunger is moving with the plunger carrier.
Needle guide
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