Preparing for Operation
Preparing the solvent and waste bottles
41
Figure 31. The maximum number of washes (pre- and post-injection)
Caution Do not exceed the solvent and waste limits of the bottles. If you exceed these
limits, sample carryover may affect your analysis.
Example 1 (tray not installed): Your application requires 10 sample
washes and 10 solvent washes with a 10-µl syringe. For three sample vials (five
injections per vial), you need 150 solvent washes, and you need to dispense 300
syringe volumes of waste. With this example, you are limited to one bottle for
solvent and one bottle for waste.
Figure 31 shows that you can have up to 250 washes from one solvent bottle
and can dispense up to 500 washes into the waste bottle. You are within the
limits and do not have to read any further.
Example 2 (tray installed): Your application requires three sample washes
and three solvent washes with a 10-µl syringe. For 40 sample vials (two
injections per vial), you need 240 solvent washes, and you need to dispense 480
syringe volumes of waste. With this example, you are using two bottles for
solvent and two bottles for waste.
Figure 31 shows that you can have up to 250 washes from each solvent bottle
and can dispense up to 1,000 washes into the waste bottles. You are within the
limits and do not have to read any further.
Example 2b (tray installed): Your application requires three sample washes
and three solvent washes with a 10-µl syringe. If you had 60 samples, you
would need 360 washes from the solvent bottles. You would have to place
Number of Bottles Solvent Limit Waste Limit
Syringe size 5 µl 10 µl 5 µl 10 µl
Two bottles 1,000 500 2,000 1,000
One bottle 500 250 1,000 500
Note: Wash volume is 0.8 times the syringe volume.
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