Preparing for Operation
Preparing the solvent and waste bottles
43
Using the equation to estimate
1. Substitute the parameters of your application into both equations.
• If you are using a 5-µl syringe, substitute 0.004 ml/wash for the
0.008 ml/wash in each equation.
• If a tray is installed or both waste bottles are being used, substitute
8.0 ml of waste for the 4.0 ml of waste in the second equation.
2. Calculate the answers for both equations. Use the smaller of the two
answers for the estimate.
• Equation S estimates the maximum number of vials from the volume
of solvent available from the bottle associated with the largest
number of washes.
Equation S
• Equation W estimates the maximum number of vials from the waste
bottle capacity.
Equation W
Equation method example
Assume a tray is installed and your application parameters are:
• Two injections per vial
• Three washes from solvent bottle A
• Two washes from solvent bottle B
• Two sample washes
• 10-µl syringe
Maximum
Number
of Vials
=
2.0 ml of solvent
0.008 ml/wash Number
injections/vial
Largest number solvent
washes from a bottle
××
Maximum
Number
of Vials
=
4.0 ml of waste
0.008 ml/wash Number
injections/vial
Number solvent and
sample washes/injection
××
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