Preparing for Operation
Preparing the solvent and waste bottles
44
1. Substitute the parameters of your application into equations S and W.
S: Maximum number of vials = 2.0 ( 0.008 x 2 x 3 ) = 41
W: Maximum number of vials = 8.0 ( 0.008 x 2 x 7 ) = 71
2. Calculate the answers for both equations. Use the smaller of the two
answers, answer (41).
Using the table to estimate
1. Go to the S table in Figure 32. In the left-hand column, find the largest
number of solvent washes you need from a solvent bottle. If you use a tray,
you can divide the solvent washes between both waste bottles.
2. Read across this row to the column with the number of injections you are
taking from each vial. The intersection of row and column is the maximum
number of vials that you can run from the limiting bottle.
• When you are using a 5-µl syringe, multiply the maximum number of
vials listed in the figure by 2.
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