Moor Instruments moorLDI2-VR - Recommendations and Protocols; Recommendations; Protocol 1

MOOR INSTRUMENTS moorLDI2 RESEARCH USER MANUAL
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2.6 RECOMMENDATIONS AND PROTOCOLS
Suggestions for protocols are based on analyses that can be obtained with the standard repeat scan
analysis and any subsequent processing by hand or within a spreadsheet etc.
2.6.1 Recommendations
1. Perform at least 2 baseline images to test for baseline stability.
2. Apply low currents (i.e. less than 100 µ A per cm
2
) to avoid the non-specific ‘galvanic effect’.
3. Using the complete protocol test for galvanic effect using blank controls i.e. vehicle (e.g.
sterilised water) alone without any dissolved drug.
4. Assess, by pilot study, the time to reach a response plateau for each drug used and in each
patient group.
5. Each scan takes a finite time so the responses at the beginning and end of a scan may be
different. To avoid problems over this reduce the scan duration (lower the image resolution)
and only take note of plateau levels whenever practical.
2.6.2 Protocol 1
Select a scan interval of 60 seconds. The total protocol duration will be 15minutes.
Scan Current
1 0
2 0
3 0
4 25
5 25
6 25
7 25
8 25
9 25
10 25
11 25
12 25
13 25
14 0
15 0
etc