Moor Instruments moorLDI2-VR - Protocol 1 Analysis; Protocol 2; Protocol 2 Analysis

MOOR INSTRUMENTS moorLDI2 RESEARCH USER MANUAL
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2.6.3 Protocol 1 Analysis
The baseline flux values can be averaged (B) and the plateau level assessed from the values of scans
11, 12 and 13 (P). The coefficient of variance (COV) is likely to be improved by expressing the
response as a percentage change: i.e. P/B x 100%
The persistence of response (R) cannot easily be assessed by imaging because of the discrete
measurement intervals. In place of this one may assess the fall off at a particular time (5 mins, say)
after stopping iontophoresis e.g. R(5) = level at 5 minutes post x 100%
P
(The above protocol could be simplified by using a 6 minute Pause instead of scans 5 to 10, because
iontophoresis is maintained at the set current during Pause. However this requires manual
intervention. The plateau can be assessed from scans 11 – 13).
2.6.4 Protocol 2
This protocol may be used to obtain a cumulative dose response curve: the suggested scan interval is
5 minutes, the total protocol duration is 45 minutes.
Scan Current
1 0
2 20
3 20
4 40
5 40
6 80
7 80
8 0
9 0
2.6.5 Protocol 2 Analysis
Analyses are similar to that of protocol 1:B, P(20), P(40), P(80) and R(5).
Assuming scan duration is small compared to response times, the P(20) value can be obtained from
scan 4, P(40) from scan 6, P(80) from scan 8 and R(5) from scan 9. B can be obtained from scans 1
and 2. This can only be done if a pilot study with more frequent scans shows that initial responses are
negligible within a single scan duration.