Moor Instruments moorLDI2-VR - 5 Introduction to Moorldi2 Software

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MOOR INSTRUMENTS moorLDI2 RESEARCH USER MANUAL

5. INTRODUCTION TO moorLDI2 SOFTWARE

Once loaded, the moorLDI2 V5.3 software presents the following options

1. MEASUREMENT To obtain Laser Doppler Measurements (scan, repeat scan, repeat line

and single point measurement)

2. IMAGE REVIEW Image processing and analysis

3. TRACE REVIEW To review single point measurements.

Main Page

Default Chapter3
- Table of Contents3
1 Safety8
- Introduction and Intended Use8
- Laser Safety8
  - LASER SAFETY Moorldi2-VR and Moorldi2-HR9
  - LASER SAFETY Moorldi2-IR and Moorldi2-HIR11
- Beam Delivery System14
- Laser Warning Labels15
- Electrical Safety Labels16
- Environmental Conditions17
- Warnings / Caution Do's and Don'ts17
2 System Description18
3 Moorldi2 Unpacking and Assembly22
- Stand Assembly22
- Unpacking the Scan Head24
- Installation of F6C Framegrabber Card (Analogue Camera Model)24
- Installation of Firewire Card (Firewire Camera Model)25
- Cable Connections26
  - Cabling26
  - Mobile Stand and Cabling33
- The Computer and Software Installation34
  - Computer Specifications34
  - Software Installation and Registration34
- Before First Use39
- Transportation39
4 The Laser Doppler Technique40
- Introduction40
- Spatial Variation of Perfusion40
- Principles of the Laser Doppler Imaging Technique40
- ELEMENTS of the Moorldi241
- The Laser Doppler Processing Algorithm42
5 Introduction to Moorldi2 Software44
- Overview45
- GETTING STARTED with the Moorldi246
- Changing Scan Parameters47
- Moorldi2-Hr and Moorldi2-Hir Scan Distance and Scan Speed47
6 Moorldi2 Set up48
- Introduction48
- Moorldi2 IMAGE SCAN CONFIGURATION49
- Scanner Set-Up49
- Setup: Preference Setup59
7 Single Image Scan61
- To Start61
- Saving an Image Scan62
- Image Display in Measurement Mode62
8 Repeat and Line Scan Measurement64
- Introduction64
- Repeat and Line Scan Set up64
  - Repeat Interval64
  - Number of Scans64
- Repeat Scan Start65
- Repeat Scan Display During Measurement66
- Repeat Line Scan Start67
- Iontophoresis67
9 Single Point Measurement (Spm)68
- Introduction68
- Positioning of Beam for Spm68
- Beam Movement68
- Beam Movement Relative to Photo Image68
- Single Point Measurement Window68
- DISTANCE - Moorldi2-TO-TISSUE69
- Single Point Configuration69
- Autogain70
- Single Point Measurement - Start70
- Single Point Measurement - File Store71
10 Image Review72
- Introduction72
- Summary of Image Processing Utilities72
- Opening Files and Image Display75
- View76
- PREFERENCE OPTIONS (for Image Review)80
11 Image Processing81
- Reload81
- Interpolate81
- Normalise81
- Rotate or Flip82
- Adjust Settings82
  - Distance82
  - Background Threshold82
- Smooth83
- Average83
- Subtract83
- Threshold/ Cut85
12 File86
- Saved Image File Formats86
- Redefine Ldi File87
- Print Report88
13 Repeat Scan Processing89
- Introduction89
- Repeat Scan Display89
- Separate Image Display90
- Re-Define Repeat File90
14 Region of Interest91
- Introduction91
- Region of Interest (Roi): Roi Toolbar91
- Defining Roi' - General92
- Zoom in92
- Polygon Roi92
- Circular Roi93
- Saving Roi93
15 Image Analysis94
- Introduction94
  - Types of Image Analysis94
  - Pixel Values94
- Statistics - Single Image Scan95
- Histogram96
- Profile99
16 Repeat Image Analysis102
- Introduction102
- Saving Results of Repeat Images106
- Repeat Line Analysis106
17 Trace Review (Single Point Measurements)108
- Introduction108
- Single Point Measurement Display108
- Trace Scrolling110
- Spm Analysis110
- Spm Trace Printout111
- Spm Statistics Printout111
- Spm Statistics - Saving Results111
18 Troubleshooting112
- Flux Image During Acquisition112
- Slow Computer Response112
- Loss of Communication with Computer113
19 Calibration and Stability115
- Moorldi V1.0 CALIBRATION GUIDE115
  - Set-Up115
  - Calibration115
- Moorldi V2.0 CALIBRATION GUIDE117
- The Distance Factor121
- Stability Testing125
20 Maintenance126
- Laser Output Power126
- Laser Beam Properties126
- Mechanical Checks126
- Operation of the Solenoid Actuated Optical Attenuator127
- Leads127
- Cleaning127
- Calibration and Stability128
21 Electromagnetic Compatability129
- Cabling129
- Guidance and Manufacturer's Declaration - Electromagnetic Emissions129
- Guidance and Manufacturers Declaration - Electromagnetic Immunity130
22 Specifications133
23 Returns Procedure137
- Decontamination137
24 Warranty and Service Policy138
- Service Due Warning138
25 Accessories139
26 Disposal140
Appendix 1. Preparing Your Moorldi2 Imager for the New Software141
Appendix 2. Moorldi/Iontophoresis User Manual V5150
- Introduction150
- INSTRUCTIONS for USE of Moorldi / IONTOPHORESIS150
- Pause Function152
- Manual Iontophoresis152
- Protocol Analysis153
- Recommendations and Protocols154
Appendix 3. Printouts from Processing156
Index161

Moor Instruments moorLDI2-VR - 5 Introduction to Moorldi2 Software

Table of Contents