MOOR INSTRUMENTS moorLDI2 RESEARCH USER MANUAL
91
Changing the lower limit will make the first bin start at the new lower limit. The graph will remain
with the origin at (0, 0).
The amplitude of the histogram (y-axis scale) can be increased using the + box or decreased using the
– box to the left of the histogram.
15.3.2 BIN NUMBER
The number of bins, ie the number of histogram sections, is either 16 or 6 if a colour palette is used.
If the grey scale is selected the bin numbers can be set by entering a number in the Bin No. Box.
After Limits or Bin number have been changed click on Plot to replot the histogram.
N.B. the flux axis (x-axis) always has 5 equal graduations marked on it and alternate graduations
annotated. For these to be easy to read and use requires a sensible choice of Upper Limit and Box No.
eg multiples of 10.
15.3.3 OTHER FEATURES
File
Save
Append
Print
Exit
The operation of the above features is described in the
statistics
section (Section 14.2).
15.3.4 SAVE HISTOGRAM RESULTS
The information
S
aved from a Histogram analysis is stored on a *.HIS ASCII file and contains
information as shown below. This file can be reviewed using a Windows
utility (e.g. Notepad) but
not using MLDI software. Note that the example is for an image recorded with a 16 colour pallette
giving a 16 Bin histogram.
moorLDI Image Histogram (WRFELBI3.FLX)
==============================================================
ROI Type: Rectangle
Lower limit = 0 Upper Limit = 2151 Total Bin No = 16
ROI No Valid Rate (%)
1 99.9
Flux Histogram (Perfusion Unit):
ROI1 35 496 836 876 745 478 248 137 84 72 32 24 12
8 9 0
To Next Page
Loading...