CHAPTER 8 Calibration
Sysmex XE-5000 Instructions for Use 8-1
Revised April 2007
8. Calibration
Calibration is performed to compensate for any inaccuracies
of the pneumatic, hydraulic, and electric systems which will
affect analysis results. This is very important in maintaining
the system accuracy.
Calibration is carried out by entering calibration values into
the unit.
Your Sysmex technical representative will perform initial
calibration of your unit at installation. After the installation, the
operator should make periodical calibration and proper quality
control to maintain the accuracy.
This chapter describes calibration procedure.
8.1 Calibration execution timing
Calibration is not required at specific intervals, but when QC
data varies with time, HGB and HCT calibration should be
checked. When abnormal QC data occur as a result of
instrument problem, reagent degradation or degeneration of
control blood, however, do not perform calibration.
8.2 Samples used for calibration
For calibration, use five to ten samples of fresh normal blood
that meets the following conditions:
• Blood of a healthy person who is not taking any medicine
• Blood added with appropriate anticoagulant
• Per-sample whole blood to exceed 2 mL
• HGB value to exceed 10.0 g/dL
• HCT value to be within 35.5% – 55.5%
8.3 Reference values
Five or more normal blood samples selected for calibration of
HGB and HCT should be analyzed accurately three times
consecutively according to the reference method:
HGB value: Cyanmethemoglobin method
HCT value: Microhematocrit method
Note:
Do not use control blood for calibration. Control blood
is specially produced for the purpose of quality control,
not for calibration.
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