CHAPTER 8 Calibration
8-6 Sysmex XE-5000 Instructions for Use
Revised April 2007
8.5 Displaying the last sample data
1. Select “Last Data” on the function menu of the Automatic
Calibration screen.
The value of last analysis sample will appear.
2. Select “OK” on the function menu of the Last Sample
Analysis Data screen. The system will return to the
Automatic Calibration screen.
8.6 Manual calibration
In manual calibration, the HGB and HCT calibration values
obtained by calculation can be entered by the numeric keys.
Calculating the calibration value
1. Analyze five to ten samples, three times each, by the
standard method to obtain average HGB and HCT values.
2. Mix the same samples sufficiently and analyze them in
manual or manual closed mode with the Main Unit (XE-
5000).
3. When there is a difference between the data obtained by
XE-5000 analysis and the reference values obtained by
the standard method, calculate new calibration values
using the following equation:
[Example]
Average of HGB values from standard method = 15.6 g/dL
Average of HGB values from XE-5000 analysis = 15.5 g/dL
Old calibration value of HGB = 100.0%
Therefore, the new calibration value of HGB needs to be
set at 100.7%. This means that the calibration value
increased 0.7%.
Current compensation rate × Reference mean
New compensation rate =
Instrument mean
100.0
15.6
15.5
-----------
× 100.65 100.7%≅×
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