CHAPTER 6 Operation
6-12 Sysmex XE-5000 Instructions for Use
Revised April 2007
6.14 Conditions for sample analysis
Sample types
Venous Blood and body fluids.
If using micro collection systems, follow the manufacturer
package inserts and use relevant standards for collecting
small samples.
Collection conditions
Blood is collected in EDTA anticoagulant. Analyze samples
within 4 hours of collection.
If it is not possible to analyze the sample within 4 hours, store
it in a refrigerator at 2-8°C until it can be analyzed.
Return a refrigerated sample to room temperature before
analyzing it (for at least 15 minutes). Next, mix the sample for
at least 2 minutes.
6.15 Analysis mode
With the XE-5000, the analysis can be performed in six
modes: manual, HPC, body fluid, capillary, sampler and
manual closed modes.
• Manual, HPC and body fluid modes
In these modes, the caps of sample tubes are manually
removed and each sample is aspirated via the whole blood
aspiration pipette.
• Capillary mode
In capillary mode, an analysis is performed after diluting the
sample to 1:5 ratio. This mode is used in analyzing a minute
amount of blood collected from the earlobe or fingertip. The
method used to aspirate the sample is the same as that
used in manual, HPC and body fluid modes.
• Sampler mode
The sampler automatically mixes, aspirates and analyzes
samples without removing their caps. Up to 100 samples
can be loaded at a time and analyzed automatically.
Note:
All performance claims given in this manual were
generated using samples in EDTA anticoagulant.
Results may be affected by the use of other
anticoagulants. Therefore, each laboratory should
develop protocols for handling samples collected in
these anticoagulants.
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