2D-LC User Guide 269
9 Data Analysis
GC Image Basic Information
Investigate the effects of using different gradients in
2
D
When combining separation systems with related separation mechanisms in the
first and second dimension (as in RPxRP), orthogonality is limited. As a result,
only a part of the available two-dimensional separation space will be occupied. In
such a case, shifted gradients in the second dimension can be used to enlarge
the accessible two-dimensional separation space.
1 To investigate the effects of using different gradients in the second
dimension, firstly run a comprehensive 2D-LC separation with the same
second dimension gradient from 5 – 95 % B repeated during the whole run.
The
1
D pump method should be set up as during the checkout runs (see
below):
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