2D-LC User Guide 97
4Installation
Software Installation
[OPTIONAL]
1 To change the Device Name, connection settings and the Pressure Units, fill in
the according fields.
2 To verify the correct
1
D and 2D pump configuration, check the Pumps
settings.
3 Select
1
D and
2
D detector under Detectors.
If necessary, it is possible to configure and select more than two detectors,
for example, an LC/MS Single Quadrupole.
The
1
D settings for the transfer volumes that determine the time between the
1
D detection of the peak and the switching of the 2D-LC Valve, depends on the
hardware setup. For a standard 2D-LC with two DADs, the transfer volume is
approx. 14 µL.
To calculate the volume, add half the volume of the detector flow cell plus the
volume between the detector flow cell and the 2D-LC Valve.
The transfer volume possibility for the
2
D detector defines the volume
between the 2D-LC Valve and the second dimension detector flow cell. This
result is set for delay calculation in 2D-LC OpenLab DA-Plugin.
2
D offset
between Sample Loop and detector ensures, that start and end of a cut
chromatogram are aligned/displayed correctly.
This action will not rename your pumps. Enter a descriptive naming during initial
instrument setup in the instrument configuration, see “Configure the HPLC
Instrument” on page 95.
The Configure 2D Pump button allows the configuration of the
2
D pump like, for
example, the solvent types.
This action will not rename your detectors. Enter a descriptive naming during
initial instrument setup in the instrument configuration, see “Configure the HPLC
Instrument” on page 95.
To determine the transfer volume (
1
D Detector to 2D-LC Valve) experimentally
more precisely, you can run a time-based High-Resolution experiment (multiple
cuts) over one of the first sample peaks. The cut with the highest abundances
then corresponds to the apex of your peak. if there is a shift of the peak to the
front or to the back, the difference in volume can be calculated and the transfer
volume adjusted.
Up to four CAN capable detectors are supported in each dimension.
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